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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Growth of the facultative chemolithoautotroph Ralstonia eutropha on organic waste materials: growth characteristics, redox regulation and hydrogenase activity

Fig. 4

ATPase activity of membrane vesicles a of R. eutropha H16 and pH dependence of 9-AA fluorescence; b and its quenching; c induced R. eutropha H16. Bacteria were grown heterotrophically in minimal salts FN or FGN media. The DCCD (0.2 mM) was added into the reaction medium where indicated. The assay of pH was the same as growth pH. The fluorescence intensity (F) in relative units at pH 6.0 was taken as 100%, and the fluorescence quenching (Q) was expressed in % of the initial value of F. Addition of 2 μM CCCP eliminated the quenching. For other details, see “Materials and methods

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