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Table 2 Lipid and biomass production in shake flasks at different C/N ratios

From: Engineering cytoplasmic acetyl-CoA synthesis decouples lipid production from nitrogen starvation in the oleaginous yeast Rhodosporidium azoricum

 

48 h

65 h

d. w.

(g/l)

Lipid titer

(g/l)

Lipid content

(g/l)

Residual ammonium

(g/l)

Residual glucose

(g/l)

d. w.

(g/l)

Lipid titer

(g/l)

Lipid content

(g/l)

Residual ammonium

(g/l)

Residual glucose

(g/l)

WT(RGRDP3)

 

 B75

7.15 ± 0.46

2.23 ± 0.36

31.2 ± 1.90

0

32.5 ± 0.25

12.9 ± 0.52

6.14 ± 0.62

52.9 ± 2.61

0

14.8 ± 1.01

 B37

7.65 ± 0.51

1.38 ± 0.45

18.1 ± 2.31

0.22 ± 0.09

31.6 ± 0.31

12.0 ± 0.43

3.45 ± 0.41

29.1 ± 1.94

0.11 ± 0.06

15.0 ± 1.12

 B20

7.57 ± 0.32

1.01 ± 0.21

15.3 ± 2.68

0.69 ± 0.08

32.9 ± 0.21

12.8 ± 0.66

3.31 ± 0.37

21.6 ± 1.98

0.55 ± 0.04

13.2 ± 0.95

ura5

 

 B75

5.27 ± 0.40

1.76 ± 0.36

33.7 ± 3.95

0

37.4 ± 0.11

5.98 ± 1.02

3.10 ± 0.57

51.8 ± 4.31

0

8.52 ± 0.18

 B37

5.70 ± 0.34

0.61 ± 0.17

10.7 ± 3.51

0.34 ± 0.03

36.3 ± 0.13

6.40 ± 0.14

1.34 ± 0.35

20.9 ± 2.45

0.08 ± 0.07

10.06 ± 0.29

 B20

5.88 ± 0.42

0.63 ± 0.37

10.6 ± 2.57

0.88 ± 0.04

39.4 ± 0.20

6.41 ± 0.39

1.07 ± 0.18

16.7 ± 2.34

0.73 ± 0.07

8.76 ± 0.15

PTAPK4

 

 B75

6.20 ± 0.27

2.46 ± 0.41

39.7 ± 3.40

0

38.8 ± 0.19

11.8 ± 0.34

8.82 ± 0.26

69.4 ± 2.32

0

3.04 ± 0.17

 B37

5.55 ± 0.38

1.66 ± 0.47

29.9 ± 2.87

0.29 ± 0.04

33.3 ± 0.12

8.70 ± 0.19

3.70 ± 0.58

42.5 ± 3.97

0.07 ± 0.05

5.91 ± 0.15

 B20

6.72 ± 0.41

1.43 ± 0.34

21.2 ± 2.99

0.82 ± 0.02

38.1 ± 0.15

8.17 ± 0.09

2.35 ± 0.34

28.7 ± 1.42

0.68 ± 0.03

6.17 ± 0.21