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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Aliivibrio wodanis as a production host: development of genetic tools for expression of cold-active enzymes

Fig. 7

a SDS-PAGE of spent growth media of A. wodanis 03/09/160. The visible protein band was analyzed by LC–MS/MS and determined to originate from AW0309160_00174. b–d Measurement of fluorescence in whole culture, growth media and periplasm of A. wodanis 03/09/160 expressing sfGFP (from plasmid pTM214_sfGFP) or a 25-aa-sfGFP fusion (from plasmid pTM214_174ss_sfGFP). Bacteria without vector was used as control/blank). e Relative secretion/translocation compared to total florescence. p value calculated with Student’s t- test

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Microbial Cell Factories

ISSN: 1475-2859

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