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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Aliivibrio wodanis as a production host: development of genetic tools for expression of cold-active enzymes

Fig. 6

Expression and purification of non-Aliivibrio “test-case” enzymes. Enzymes from a wider phylogenetic range were selected as test-cases. a MvExoI (Mw—55 kDa), Exonucleae I from Moritella viscosa. b AdhStrep (Mw—39 kDa), alkoholdehydrogenase from Streptomyces. c CpLig6 (Mw—32 kDa), Ligase 6 from Colwellia psychrerythraea. d CsLig1 (Mw—66 kDa), Ligase 1 from Cenarchaeum symbiosum. Arrowheads indicate bands of expected size. MW = molecular weight marker. WC = whole cell extract, Sol = lysate soluble protein fraction. Immobilized metal affinity chromatography (IMAC) was done to purify 6 × His-tagged proteins, and proteins eluted in IMAC fractions are shown on the gels

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