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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Aliivibrio wodanis as a production host: development of genetic tools for expression of cold-active enzymes

Fig. 4

Expression, purification and activity of AsExoI and AsPolII. Coomassie-stained SDS acrylamide gels showing expressed and affinity purified 6 × His-AsExoI (a) and 6 × His-AsPolII (b). MW = molecular weight marker (Biorad protein standard), WC = whole cell extract, Sol = lysate soluble protein fraction. Immobilized metal affinity chromatography (IMAC) was done to purify 6 × His-tagged proteins, and proteins eluted in IMAC fractions are shown on the gels. Molecular weights of AsExoI ans AsPolII are theoretically 55.0 kDa and 91.4 kDa. Arrows heads indicate bands on geld that were identified as the desired enzyme targets. The activity of AsExoI (c) and AsPolII (d) was monitored by adding increasing concentrations of enzyme to molecular beacon substrate

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