Fig. 5
From: High production of valencene in Saccharomyces cerevisiae through metabolic engineering
Overexpression of FPP synthetic pathway and the yield determination of overexpression strain. a 8 genes in FPP synthetic pathway were overexpressed in yeast genome using recyclable CRISPR/Cas9 system. Four gene loci (bts1, rox1, dpp1, lpp1) were chosen for integration of FPP synthetic genes. b The fermentation result in shake flask detected by GC–MS. OF* stands for overexpression of FPP synthetic pathway