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Table 3 Residual protease activity of recombinant pernisine in the presence of reducing agents, denaturants, and a detergent

From: Extracellular production of the engineered thermostable protease pernisine from Aeropyrum pernix K1 in Streptomyces rimosus

Reagent

Concentration

Residual proteinase activity at 1 mM CaCl2 (%)

Present study

Recombinant pernisine in E. colia

Native pernisineb

None

100.0 ± 5.3

100 ± 4.7

100.0

DTT

1 mM

79.3 ± 4.0

58.0 ± 2.3

27.9

 

5 mM

63.7 ± 1.8

47.9 ± 4.5

25.9

2-MeEtOH

1%

78.5 ± 7.6

50.6 ± 11.6

34.8

 

5%

63.1 ± 2.6

38.4 ± 2.5

30.8

Gdn-HCl

1 M

218.6 ± 24.7

124.9 ± 4.0

66.2

 

4 M

189.0 ± 12.3

189.6 ± 10.1

152.2

Urea

1 M

136.0 ± 9.0

119.7 ± 9.7

51.7

 

4 M

121.8 ± 9.7

106.0 ± 3.1

46.8

SDS

0.10%

107.0 ± 8.1

90.8 ± 0.8

65.7

 

3%

22.3 ± 3.3

10.4 ± 2.8

33.8

  1. DTT dithiothreitol, 2-MeEtOH 2-mercaptoethanol, Gdn-HCl guanidinium hydrochloride, SDS sodium dodecil sulphate
  2. aŠnajder et al. [7]
  3. bŠnajder et al. [6]