Reagent | Concentration | Residual proteinase activity at 1 mM CaCl2 (%) | ||
---|---|---|---|---|
Present study | Recombinant pernisine in E. colia | Native pernisineb | ||
None | – | 100.0 ± 5.3 | 100 ± 4.7 | 100.0 |
DTT | 1 mM | 79.3 ± 4.0 | 58.0 ± 2.3 | 27.9 |
5 mM | 63.7 ± 1.8 | 47.9 ± 4.5 | 25.9 | |
2-MeEtOH | 1% | 78.5 ± 7.6 | 50.6 ± 11.6 | 34.8 |
5% | 63.1 ± 2.6 | 38.4 ± 2.5 | 30.8 | |
Gdn-HCl | 1 M | 218.6 ± 24.7 | 124.9 ± 4.0 | 66.2 |
4 M | 189.0 ± 12.3 | 189.6 ± 10.1 | 152.2 | |
Urea | 1 M | 136.0 ± 9.0 | 119.7 ± 9.7 | 51.7 |
4 M | 121.8 ± 9.7 | 106.0 ± 3.1 | 46.8 | |
SDS | 0.10% | 107.0 ± 8.1 | 90.8 ± 0.8 | 65.7 |
3% | 22.3 ± 3.3 | 10.4 ± 2.8 | 33.8 |