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Table 1 Expression vectors used in this study

From: Extracellular production of the engineered thermostable protease pernisine from Aeropyrum pernix K1 in Streptomyces rimosus

Vector

Protein assembly

Theoretical MW (kDa)

Proregion

S. rimosus signal sequence

Codon optimized

pVFPER1

Prepropernisine-His6-C

43

Yes

No

No

pVFPER2

Prepropernisine-His6-C

43

Yes

No

Yes

pVFPER3

srT-propernisine-His6-C

45

Yes

Yes

Yes

pVFPER4

Pernisine-His6-C

36

No

No

Yes

pVFPER5

srT-pernisine-His6-C

38

No

Yes

Yes

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Microbial Cell Factories

ISSN: 1475-2859

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