Fig. 7
Western blotting with Prionics–Check Western kit (PCW) showing degradation of bovine-brain homogenate containing normal cellular prion protein (PrPc) (a, lanes 1–6) and infectious prion protein (PrPSc) (b, lanes 1–6) by the codon-optimised recombinant pernisine (from plasmid construct pVFPER5) from S. rimosus (lanes 1–6), and at different dilutions of the brain homogenates in the reaction mixtures. PCW brain homogenate, undiluted (lane 1), and PCW brain homogenate diluted with water (v/v) 1:1 (lane 2), 1:2 (lane 3), 1:3 (lane 4), 1:4 (lane 5) and 1:5 (lane 6). Controls to the codon-optimised recombinant pernisine: untreated control (a, lane K); wild-type pernisine (a, b, lane 7; brain homogenate dilution 1:1); proteinase K from the kit (a, b, lane 8; brain homogenate dilution 1:1)