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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Extracellular production of the engineered thermostable protease pernisine from Aeropyrum pernix K1 in Streptomyces rimosus

Fig. 4

Analysis of the isolated and purified codon-optimised pernisine produced by S. rimosus transformants transformed with plasmid construct pVFPER5, using SDS-PAGE (a) and zymography (b). Purified codon-optimised pernisine was visualised on standard 15% SDS-PAGE (a) and 15% SDS-PAGE with casein as substrate (b) for the zymography activity (2 h at 80 °C). Lane 0, molecular weight markers (indicated left); Lane 1, cell lysate fraction; Lane 2, fraction from Ni–NTA chromatography; Lane 3, fraction from preparative grade gel filtration column (Superdex 200); Lane 4, dialysed codon-optimised pernisine fraction

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Microbial Cell Factories

ISSN: 1475-2859

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