Fig. 3From: Extracellular production of the engineered thermostable protease pernisine from Aeropyrum pernix K1 in Streptomyces rimosusCodon optimised srT-propernisine (plasmid construct pVFPER3) in the intracellular and extracellular cell fractions following purification using affinity chromatography and analysis by SDS-PAGE (a) and zymography (b). Lanes 0, protein molecular weight markers; lanes 1, intracellular codon-optimised srT-pernisine; lanes 2, extracellular codon-optimised srT-pernisineBack to article page