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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Extracellular production of the engineered thermostable protease pernisine from Aeropyrum pernix K1 in Streptomyces rimosus

Fig. 3

Codon optimised srT-propernisine (plasmid construct pVFPER3) in the intracellular and extracellular cell fractions following purification using affinity chromatography and analysis by SDS-PAGE (a) and zymography (b). Lanes 0, protein molecular weight markers; lanes 1, intracellular codon-optimised srT-pernisine; lanes 2, extracellular codon-optimised srT-pernisine

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