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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Extracellular production of the engineered thermostable protease pernisine from Aeropyrum pernix K1 in Streptomyces rimosus

Fig. 2

Analysis using SDS-PAGE (a), zymography (b) and Western blotting (c) following the initial trial for activation of prepropernisineCO from total cell lysates of S. rimosus transformed with plasmid construct pVFPER2. Lane 0, molecular weight markers; Lane 1, prepropernisine in 10 mM HEPES, pH 8.0, 1 mM CaCl2; Lane 2, heat-activated prepropernisine (30 min, 80 °C). Processed prepropernisine bands are indicated with arrows

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Microbial Cell Factories

ISSN: 1475-2859

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