Table 4 Biobricks used in this study
BioBrick ID | Template for PCR | Fw primer | Rv primer |
|---|---|---|---|
BB0380 (Fj_tal<-) | F. johnsoniaeu codon-optimized synthetic gene | Fj_Tal_U1_fw (ID1691) | Fj_TalU1_rv (ID1692) |
BB254 (KlLEU2_2/3_START) | p0019(pUG73) | Sc_LEU2_2/3_START_fw (ID476) | Sc_LEU2_2/3_START_rv (ID150) |
BB251 | CEN.PK113-7D gDNA | Sc_Pdc5_UP_fw (ID1368) | Sc_Pdc5_UP_rv (ID1369) |
BB252 | CEN.PK113-7D gDNA | Sc_Pdc5_END_fw (ID1370) | Sc_Pdc5_END_fw (ID1371) |
BB681 | CEN.PK113-7D gDNA | Sc_Aro10_UP_fw (1372) | Sc_Aro10_UP_rv (1373) |
BB0501 (Ec_AroL->) | EcoMG1655 ATCC 31884 gDNA | Ec_AroL_U2_fw (ID6785) | Ec_AroL_U2_rv (ID6786) |
BB0361 (Sc_Aro7_G141S<-) | p0761 (pESC-URA-ARO7pm) | Sc_aro7_U1_fw (ID1398) | Sc_aro7_U1_rv (ID1399) |
BB0364 (Sc_Aro4_K229L->) | p0775 (pESC-HIS-ARO4pm) | Sc_aro4_U2_fw (ID1396) | Sc_aro4_U2_rv (ID1397) |
BB1189 (Pdc5_Up_kanmx_2/3 start) | p0015 (pUG6) | Pdc5_UP_fw (ID1368) | URA3_2/3_START_rv (ID92) |
BB1190(kanmx_2/3_end_Pdc5_down) | p0015 (pUG6) | URA3_2/3_END_fw (ID93) | Pdc5_END_rv (ID1371) |
BB828 (aro10_UP_Leu2_2/3_start) | BB251, BB245 | Sc_Aro10_UP_fw (1372) | Sc-LEU2_2/3_START_rv (ID150) |
BB829 (Leu2_2/3_end_aro10_down) | BB681, BB252 | LEU2_2/3_END_fw (ID151) | Aro10_END_rv (ID1375) |