|
Overexpression primers
|
|
1691
|
Fj_TAL_1_fw
|
AGTGCAGGUAAAACAATGAACACCATCAACGAATATCTGAGC
|
|
1692
|
Fj_TAL_1_rv
|
CGTGCGAUTTAATTGTTAATCAGGTG
|
|
6785
|
Ec_aroL_2_fw
|
ATCTGTCAUAAAACAATGACACAACCTCTTTTTCTGA
|
|
6786
|
Ec_aroL_2_rv
|
CACGCGAUTCAACAATTGATCGTCTGTGC
|
|
1398
|
Sc_ARO7_1_fw
|
AGTGCAGGUAAAACAATGGATTTCACAAAACCAGAAAC
|
|
1399
|
Sc_ARO7_1_rv
|
CGTGCGAUTCACTCTTCCAACCTTCTTAGCAAG
|
|
1396
|
Sc_ARO4_2_fw
|
ATCTGTCAUAAAACAATGAGTGAATCTCCAATGTTCG
|
|
1397
|
Sc_ARO4_2_rv
|
CACGCGAUTCATTTCTTGTTAACTTCTCTTCTTTG
|
|
Verification primers
|
|
904
|
Sc_X-3-out-seq_rv
|
CCGTGCAATACCAAAATCG
|
|
906
|
Sc_X-4-out-seq_rv
|
GACGGTACGTTGACCAGAG
|
|
912
|
XI-3- down-out_rv
|
CACATTGAGCGAATGAAACG
|
|
2220
|
Sc_ColoPCR_fw
|
CCTGCAGGACTAGTGCTGAG
|
|
1384
|
Sc_PDC5_Start_fw
|
AAAGCCTCCATATCCAAAG
|
|
1385
|
Sc_PDC5_End_rv
|
AGGTATGGTTAAAGATCACAC
|
|
1386
|
Sc_ARO10_Start_fw
|
ACCGAAATTTAAAAAAGCAG
|
|
1387
|
Sc_ARO10_End_rv
|
GTTTTCGGATAAAACTTCTTC
|
|
Knockout primers
|
|
1368
|
Pdc5 _UP_fw
|
CGTAAACCTGCATTAAG
|
|
1369
|
Pdc5 _UP_rv
|
GATCCCCGGGAATTGCCATTGTGTTGTTCTCTTTG
|
|
1370
|
Pdc5_END_fw
|
GGTACCCAATTCGCCCTAGATTCAACGTTTGTGTA
|
|
1371
|
Pdc5_END_rv
|
CTAAGATCATAGCTAAAGG
|
|
1372
|
Aro10_UP_fw
|
GGATAGCCGTCATTTAC
|
|
1373
|
Aro10_UP_rv
|
GATCCCCGGGAATTGCCAGAGGGTTGATCAGTTAAA
|
|
1374
|
Aro10_END_fw
|
GGTACCCAATTCGCCCTACTACCAATTGTTCGTTT
|
|
1375
|
Aro10_END_rv
|
CGATAGGAATGACAGAA
|
|
476
|
KanMX_UP_fw
|
TGGCAATTCCCGGGGATCACGCTGC AGGTCGACAAC
|
|
477
|
KanMX_UP_rv
|
AGTGACGACTGAATCCGGTG
|
|
478
|
KanMX_END_fw
|
AATGGGCTCGCGATAATGTC
|
|
479
|
KanMX_END_rv
|
TAGGGCGAATTGGGTACCGCCACTAGTGGA TCTGATATCAC
|
|
150
|
LEU_UP_rv
|
CAGAAGCATAACTACCCATTCC
|
|
151
|
LEU_END_Fw
|
TGGAAGAGGCAAGCACGTTAGC
|
|
92
|
URA3_2/3_START_rv
|
CGCTTCCCATCCAGCATTTC
|
|
93
|
URA3_2/3_END_fw
|
CTGTCGTTCCATTGAAAGC
|
