Table 3 Primers used in this study
ID | Name | Sequence |
|---|---|---|
Overexpression primers | ||
 1691 | Fj_TAL_1_fw | AGTGCAGGUAAAACAATGAACACCATCAACGAATATCTGAGC |
 1692 | Fj_TAL_1_rv | CGTGCGAUTTAATTGTTAATCAGGTG |
 6785 | Ec_aroL_2_fw | ATCTGTCAUAAAACAATGACACAACCTCTTTTTCTGA |
 6786 | Ec_aroL_2_rv | CACGCGAUTCAACAATTGATCGTCTGTGC |
 1398 | Sc_ARO7_1_fw | AGTGCAGGUAAAACAATGGATTTCACAAAACCAGAAAC |
 1399 | Sc_ARO7_1_rv | CGTGCGAUTCACTCTTCCAACCTTCTTAGCAAG |
 1396 | Sc_ARO4_2_fw | ATCTGTCAUAAAACAATGAGTGAATCTCCAATGTTCG |
 1397 | Sc_ARO4_2_rv | CACGCGAUTCATTTCTTGTTAACTTCTCTTCTTTG |
Verification primers | ||
 904 | Sc_X-3-out-seq_rv | CCGTGCAATACCAAAATCG |
 906 | Sc_X-4-out-seq_rv | GACGGTACGTTGACCAGAG |
 912 | XI-3- down-out_rv | CACATTGAGCGAATGAAACG |
 2220 | Sc_ColoPCR_fw | CCTGCAGGACTAGTGCTGAG |
 1384 | Sc_PDC5_Start_fw | AAAGCCTCCATATCCAAAG |
 1385 | Sc_PDC5_End_rv | AGGTATGGTTAAAGATCACAC |
 1386 | Sc_ARO10_Start_fw | ACCGAAATTTAAAAAAGCAG |
 1387 | Sc_ARO10_End_rv | GTTTTCGGATAAAACTTCTTC |
Knockout primers | ||
 1368 | Pdc5 _UP_fw | CGTAAACCTGCATTAAG |
 1369 | Pdc5 _UP_rv | GATCCCCGGGAATTGCCATTGTGTTGTTCTCTTTG |
 1370 | Pdc5_END_fw | GGTACCCAATTCGCCCTAGATTCAACGTTTGTGTA |
 1371 | Pdc5_END_rv | CTAAGATCATAGCTAAAGG |
 1372 | Aro10_UP_fw | GGATAGCCGTCATTTAC |
 1373 | Aro10_UP_rv | GATCCCCGGGAATTGCCAGAGGGTTGATCAGTTAAA |
 1374 | Aro10_END_fw | GGTACCCAATTCGCCCTACTACCAATTGTTCGTTT |
 1375 | Aro10_END_rv | CGATAGGAATGACAGAA |
 476 | KanMX_UP_fw | TGGCAATTCCCGGGGATCACGCTGC AGGTCGACAAC |
 477 | KanMX_UP_rv | AGTGACGACTGAATCCGGTG |
 478 | KanMX_END_fw | AATGGGCTCGCGATAATGTC |
 479 | KanMX_END_rv | TAGGGCGAATTGGGTACCGCCACTAGTGGA TCTGATATCAC |
 150 | LEU_UP_rv | CAGAAGCATAACTACCCATTCC |
 151 | LEU_END_Fw | TGGAAGAGGCAAGCACGTTAGC |
 92 | URA3_2/3_START_rv | CGCTTCCCATCCAGCATTTC |
 93 | URA3_2/3_END_fw | CTGTCGTTCCATTGAAAGC |