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Table 2 Physiological parameters for batch and chemostat cultivation

From: Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose

 

Substrate

Glucose

Xylose

Batch

  

 µmax (h −1)

0.32 ± 0.02

0.11 ± 0.01

 Biomass concentration (Cx in gDW/L)

4.5 ± 0.8

12.16 ± 0.9

 p-coumaric acid (mg/L)

5.35 ± 0.32

242 ± 12

 YSX (g/gDW)

0.13 ± 0.02

0.5 ± 0.08

 YPX (mg/gDW)

1.18 ± 0.12

19.90 ± 2

 YPS (mg/gDW)

0.214 ± 0.02

9.68 ± 0.59

 qs (g gDW/h)

2.46 ± 0.43

0.23 ± 0.02

 qgly (g/gDW h)

0.10 ± 0.02

0.001 ± 3 × 10−4

 qeth (g/gDW h)

1.37 ± 0.38

0.002 ± 1 × 10−4

 qac (g/gDW h)

0.1 ± 0.02

1 × 10−4 ± 1 × 10−5

 qp (g/gDW h)

0.38 ± 0.02

2.19 ± 0.31

 Qp (g/gDW/h)

0.22 ± 0.02

4.75 ± 0.45

 qO2 (mmol/C-mmol/h)

11.1 ± 0.6

0.96 ± 0.07

 qCO2 (mmolCO2/C-mmol/h)

15.02 ± 0.78

1.06 ± 0.09

 RQ (–)

0.70

1.11

Chemostat

 

 D (h−1)

0.048 ± 0.003

0.047 ± 0.002

 Feeding solution (Cx in g/L)

7.5

15

 Biomass concentration (Cx in gDW/L)

2.87 ± 0.3

3.62 ± 0.3

 Residual substrate (g/L)

ND

7.66 ± 0.3

 p-coumaric acid (mg/L)

2.23 ± 0.04

55.5 ± 3

 qs (mmol/gDW/h)

1.41 ± 0.09

0.55 ± 0.02

 qgly (mmol/gDW/h)

2.54E−03

ND

 qeth (mmol/gDW/h)

1.70E−01

ND

 qac (mmol/gDW/h)

2.84E−03

ND

 qO2 (mmol/gDW/h)

0.25 ± 0.02

0.11 ± 0.02

 qCO2 (mmolCO2/gDW/h)

0.22 ± 0.22

0.09 ± 0.02

 RQ (–)

1.13 ± 0.03

1.22 ± 0.03

 Dissolved oxygen (%)

> 80

> 80

  1. Data are means from four independent fermentations (n = 4 ± standard deviation, sd)
  2. RQ respiratory quotient, ND not detected