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Table 2 Physiological parameters for batch and chemostat cultivation

From: Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose

  Substrate
Glucose Xylose
Batch   
 µmax (h −1) 0.32 ± 0.02 0.11 ± 0.01
 Biomass concentration (Cx in gDW/L) 4.5 ± 0.8 12.16 ± 0.9
 p-coumaric acid (mg/L) 5.35 ± 0.32 242 ± 12
 YSX (g/gDW) 0.13 ± 0.02 0.5 ± 0.08
 YPX (mg/gDW) 1.18 ± 0.12 19.90 ± 2
 YPS (mg/gDW) 0.214 ± 0.02 9.68 ± 0.59
 qs (g gDW/h) 2.46 ± 0.43 0.23 ± 0.02
 qgly (g/gDW h) 0.10 ± 0.02 0.001 ± 3 × 10−4
 qeth (g/gDW h) 1.37 ± 0.38 0.002 ± 1 × 10−4
 qac (g/gDW h) 0.1 ± 0.02 1 × 10−4 ± 1 × 10−5
 qp (g/gDW h) 0.38 ± 0.02 2.19 ± 0.31
 Qp (g/gDW/h) 0.22 ± 0.02 4.75 ± 0.45
 qO2 (mmol/C-mmol/h) 11.1 ± 0.6 0.96 ± 0.07
 qCO2 (mmolCO2/C-mmol/h) 15.02 ± 0.78 1.06 ± 0.09
 RQ (–) 0.70 1.11
Chemostat  
 D (h−1) 0.048 ± 0.003 0.047 ± 0.002
 Feeding solution (Cx in g/L) 7.5 15
 Biomass concentration (Cx in gDW/L) 2.87 ± 0.3 3.62 ± 0.3
 Residual substrate (g/L) ND 7.66 ± 0.3
 p-coumaric acid (mg/L) 2.23 ± 0.04 55.5 ± 3
 qs (mmol/gDW/h) 1.41 ± 0.09 0.55 ± 0.02
 qgly (mmol/gDW/h) 2.54E−03 ND
 qeth (mmol/gDW/h) 1.70E−01 ND
 qac (mmol/gDW/h) 2.84E−03 ND
 qO2 (mmol/gDW/h) 0.25 ± 0.02 0.11 ± 0.02
 qCO2 (mmolCO2/gDW/h) 0.22 ± 0.22 0.09 ± 0.02
 RQ (–) 1.13 ± 0.03 1.22 ± 0.03
 Dissolved oxygen (%) > 80 > 80
  1. Data are means from four independent fermentations (n = 4 ± standard deviation, sd)
  2. RQ respiratory quotient, ND not detected