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Table 1 Xylanase overexpression and enhanced extracellular protein secretion in PFIX7

From: Redesigning the Aspergillus nidulans xylanase regulatory pathway to enhance cellulase production with xylose as the carbon and inducer source

 

Inducer

Xylanase

Total protein

CbhC

Xylose (%)

U (µmol/µl/min)a

µg/ml

U (pNPC)b

WT

2

505 ± 70

259 ± 78

0.63 ± 0.29

4

628 ± 85

260 ± 39

0.36 ± 0.06

6

593 ± 32

302 ± 32

0.45 ± 0.03

PFIX7

2

14,023 ± 4329

1442 ± 349

4.23 ± 0.06

4

16,248 ± 3091

1176 ± 233

4.01 ± 0.02

6

14,958 ± 2746

1225 ± 211

2.59 ± 0.01

  1. aSubstrate was beechwood xylan; b substrate was pNPC (p-nitrophenyl β-d-cellobioside)
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Microbial Cell Factories

ISSN: 1475-2859

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