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Table 3 Comparison of Crl1 production-related parameters for fed-batch cultivations

From: Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage

  Single-copy clone Multi-copy clone
µ (h−1) 0.028 0.047 0.084 0.027 0.046 0.063
Product titer (AU mL−1) 769 660 261 1386 1542 1033
Product titer ratio 1.80 2.32 3.95
qP (AU g −1X h−1) 364 460 326 628 1322 958
qP ratio 1.73 2.25 2.93
QP (kAU L−1 h−1) 7.87 10.89 5.53 14.66 21.81 18.01
QP ratio 1.86 2.00 3.26
YP/S (kAU g −1S ) 5.37 4.67 1.86 9.74 12.19 7.25
YP/S ratio 1.81 2.74 3.90
YP/X (kAU g −1X ) 13.16 9.76 3.91 23.84 28.88 15.32
YP/X ratio 1.81 2.56 3.91
  1. The ratios between the MCC and SCC were calculated by dividing the MCC parameter values to SCC ones at similar specific growth rate (µ)