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Table 2 Comparison of Crl1 production-related parameters for chemostat cultivations

From: Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage

  Single copy clone Multi copy clone
D (h−1) 0.019 0.035 0.050 0.064 0.079 0.095 0.020 0.036 0.050 0.066 0.079 0.097
Product titer (AU mL−1) 124 101 118 107 93 68 321 267 274 243 212 155
Product titer ratio 2.59 2.64 2.32 2.27 2.28 2.28
qP (AU g −1X h−1) 132 183 306 346 352 312 297 449 646 735 751 656
qP ratio 2.25 2.45 2.11 2.12 2.13 2.10
QP (kAU L−1 h−1) 2.43 3.51 5.91 7.59 7.31 6.51 6.46 9.81 13.72 16.02 16.71 15.15
QP ratio 2.66 2.79 2.32 2.11 2.29 2.33
YP/S (kAU g −1S ) 2.50 1.96 2.45 2.17 1.92 1.41 6.31 5.52 5.41 4.79 4.05 3.01
YP/S ratio 2.52 2.8 2.21 2.21 2.11 2.13
YP/X (kAU g −1X ) 6.76 5.25 6.09 4.88 4.48 3.27 15.77 13.08 12.92 11.18 9.55 6.74
YP/X ratio 2.33 2.49 2.12 2.29 2.13 2.06
  1. The ratios between the MCC and SCC were calculated by dividing the MCC parameter values to SCC ones at similar dilution rate (D)