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Table 2 Comparison of Crl1 production-related parameters for chemostat cultivations

From: Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage

 

Single copy clone

Multi copy clone

D (h−1)

0.019

0.035

0.050

0.064

0.079

0.095

0.020

0.036

0.050

0.066

0.079

0.097

Product titer (AU mL−1)

124

101

118

107

93

68

321

267

274

243

212

155

Product titer ratio

2.59

2.64

2.32

2.27

2.28

2.28

qP (AU g −1X h−1)

132

183

306

346

352

312

297

449

646

735

751

656

qP ratio

2.25

2.45

2.11

2.12

2.13

2.10

QP (kAU L−1 h−1)

2.43

3.51

5.91

7.59

7.31

6.51

6.46

9.81

13.72

16.02

16.71

15.15

QP ratio

2.66

2.79

2.32

2.11

2.29

2.33

YP/S (kAU g −1S )

2.50

1.96

2.45

2.17

1.92

1.41

6.31

5.52

5.41

4.79

4.05

3.01

YP/S ratio

2.52

2.8

2.21

2.21

2.11

2.13

YP/X (kAU g −1X )

6.76

5.25

6.09

4.88

4.48

3.27

15.77

13.08

12.92

11.18

9.55

6.74

YP/X ratio

2.33

2.49

2.12

2.29

2.13

2.06

  1. The ratios between the MCC and SCC were calculated by dividing the MCC parameter values to SCC ones at similar dilution rate (D)