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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1

Fig. 1

Comparison of amino acid sequences of Lp_2757 and Lp_0179 GH13 α-amylases from L. plantarum WCFS1. Aligment was done using the program ClustalOmega. Residues that are identical (*), conserved (:) or semiconserved (.) in both sequences are indicated. Dashes indicated gaps introduced to maximize similarities. The GH13 three domains are highlighted in blue (domain A), green (domain B), and yellow (domain C). The N-terminal domain of Lp_2757 (CBM34) is indicated in dark blue. The seven conserved sequence regions (CSR) found in GH13_20 subfamily α-amylases are also indicated. The residues of the GH13 catalytic triad (Asp-171, Glu-200, and Asp-227 in Lp_0179) are highlighted in pink colour. Conserved residues in amylolytic enzymes are highlighted in red (Lee et al., 2002). The conserved VAnE and MpKln motifs, in the CSR II and V respectively, are indicated in grey colour

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