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Table 1 Catalytic power of the MEP pathway enzymes in E. coli

From: Investigation of the methylerythritol 4-phosphate pathway for microbial terpenoid production through metabolic control analysis

Enzyme kcat (103 min−1) References for kcat Amount per cell Maximum reaction rate per cell (Vmax) (103 molecules min−1 cell−1)
Dxs 0.25 [41] 64 ± 4 16
Dxr 1.3; 6.7; 5.9 × 102 [42,43,44] 32 ± 5 41; 2.1 × 102; 1.8 × 104
IspD 2.94 [47] 50 ± 8 1.48 × 102
IspE 1.01b [48] 208 ± 22 2.1 × 102
IspF 0.06   111 ± 13 6.66
IspG 0.024a [49] 204 ± 25 4.83
IspH 1.13a [50] 218 ± 22 2.46 × 102
Idi 0.0048–0.02 [36, 51] < 10 < 0.2
  1. a Kinetics were partly determined using artificial reducing agents
  2. b Calculated from a Vmax of 162.9 nmol min−1 for 5 µg pure IspE and using 31 kDa as the molecular weight of IspE