Table 4 Effect of protein denaturants and EDTA on the activities of purified ReGal2
Compound | 0 mM | 50 mM | 100 mM | 200 mM |
|---|---|---|---|---|
SDS | 100 ± 5.12 | 106 ± 4.7 | 103 ± 6.8 | 104 ± 2.39 |
Urea | 100 ± 0.26 | 99 ± 2.0 | 105 ± 2.9 | 109 ± 1.3 |
GdnHCl | 100 ± 4.3 | 88 ± 2.6 | 80 ± 0.8 | 68 ± 2.3 |
EDTA | 100 ± 5.2 | 102 ± 4.02 | 104 ± 2.98 | 100 ± 5.65 |
β-mercaptoethanol | 100 ± 1.87 | 96 ± 1.69 | 104 ± 2.61 | 106 ± 4.75 |