Characterization of a highly stable α-galactosidase from thermophilic Rasamsonia emersonii heterologously expressed in a modified Pichia pastoris expression system

Table 2 Optimum temperatures of heterologously expressed ReGal2–ReGal5

Name	NCBI protein accession no.	Length (amino acids)	GH family	Optimum temperature (°C)	pNPGal activity (U ml⁻¹)
ReGal2	XP_013324753.1	491	27	80	38.0
ReGal3	XP_013323553.1	607	27	50	20.7
ReGal4	XP_013332284.1	545	27	50	11.2
ReGal5	XP_013330325.1	682	27	65	3.5

The pNPGal hydrolytic activities and the optimal temperatures were determined with crude culture supernatant of P. pastoris transformants

ISSN: 1475-2859