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Table 1 Comparison of enzymatic properties of ReGal2 with other thermophilic fungal GH27 α-galactosidases

From: Characterization of a highly stable α-galactosidase from thermophilic Rasamsonia emersonii heterologously expressed in a modified Pichia pastoris expression system

Organism Optimal pH Optimal temperature/thermostability Km (mM) Vmax (μmol min−1 mg−1) kcat (s−1) Kcat/Km (s−1mM−1) References
Talaromyces emersonii 4.0 80 °C/70 °C for 60 h 0.19 566.5 623.15 3279.7 This study
T. leycettanus JCM12802 4.0 70 °C/60% at 65 °C for 1 h 1.32 389.8 341 258.4 [12]
Neosartorya fischeri P1 4.0 75 °C/60 °C for 1 h 2.84 1850 1621 570.8 [10]
Talaromyces emersonii 4.5 70 °C/50 °C for 10 days 0.29 240.3 200.3 690.5 [13]
Candida javanica 4.0 70 °C/70% at 70 °C for 15 min 11 100 [36]
Thermomyces lanuginosus 4.5–5.0 65–70 °C/60 °C for 6 h 0.5 52.4 49.8 97.8 [11]
Aspergillus terreus 5.0 65 °C/65 °C for 30 min 0.11 7.2 [37]
Neosartorya fischeri P1 4.5 60 °C/60 °C for 10 min 0.8 449.5 368.6 460.8 [9]
Penicillium canescens 4.0–5.0 55 °C/40–50 °C for 3 h 0.48 6.89 7 14.6 [38]
Agaricus bisporus 4.0 60 °C/– 0.30 193.12 [39]
Rhizomucor miehei 4.5 60 °C/55 °C for 30 min 0.36 378.9 6.32 17.5 [32]
Irpex lacteus 4.8 70 °C/90% at 60 °C for 10 h 1.2 1012 1900 [40]