Fig. 4

ReGal2 is an oligomer. a SDS-PAGE analysis of purified ReGal2 with (lane 1) or without (lane 2) boiling for 15 min. Samples loaded were routinely pretreated with SDS and β-mercaptoethanol. b Semi-native PAGE of purified ReGal2 treated with (lane 1) or without (lane 2) 15-min boiling as shown in a for determination of its hydrolytic activity toward MUG. c Determination of the molecular weight of purified ReGal2 by gel filtration chromatography using a calibration curve plotted with elution volume versus commonlogarithm of protein molecular mass