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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Characterization of a highly stable α-galactosidase from thermophilic Rasamsonia emersonii heterologously expressed in a modified Pichia pastoris expression system

Fig. 4

ReGal2 is an oligomer. a SDS-PAGE analysis of purified ReGal2 with (lane 1) or without (lane 2) boiling for 15 min. Samples loaded were routinely pretreated with SDS and β-mercaptoethanol. b Semi-native PAGE of purified ReGal2 treated with (lane 1) or without (lane 2) 15-min boiling as shown in a for determination of its hydrolytic activity toward MUG. c Determination of the molecular weight of purified ReGal2 by gel filtration chromatography using a calibration curve plotted with elution volume versus commonlogarithm of protein molecular mass

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