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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Characterization of a highly stable α-galactosidase from thermophilic Rasamsonia emersonii heterologously expressed in a modified Pichia pastoris expression system

Fig. 3

Markedly increased ReGal2 production in the optimized P. pastoris expression system with enhanced AOX1 promoter activity. a Schematic illustration of the relevant cis-elements within the native AOX1 promoter and the engineered MBS-AOX1 promoter. b Evaluation of the AOX1 promoter activity in the conventional or engineered P. pastoris expression system by determining the fluorescence intensity from the EGFP reporter protein. c Schematic illustration of the construction of the optimum engineered P. pastoris expression system that contains both MBS-AOX1 promoter and overexpressed Mxr1. d Extracellular pNPGal hydrolytic activity of the culture supernatant from the conventional and the engineered P. pastoris cells expressing ReGal2, respectively. e SDS-PAGE analysis of the 120 h-culture supernatant of the conventional (lane 1) and the engineered P. pastoris cells (lane 2) expressing ReGal2, respectively. Samples was pretreated with SDS, β-mercaptoethanol and 5-min boiling according to standard protocols before being loaded for SDS-PAGE analysis

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Microbial Cell Factories

ISSN: 1475-2859

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