Fig. 2

Heterologous production of ReGal2 to ReGal5 in P. pastoris GS115 and determination of their optimum temperatures. a SDS-PAGE analysis of the crude culture supernatant of P. pastoris GS115 transformants expressing ReGal2 to ReGal5, respectively. b–e Determination of the optimum temperatures of ReGal2 (b), ReGal3 (c), ReGal4 (d), and ReGal5 (e) with the crude culture supernatant of respective P. pastoris transformants after 0.5% methanol induction