Fig. 4
Peroxidase activities of MstX. a–c The coupled assay of the MstX/MSH/Mtr/NADPH pathway with H2O2, CHP, and t-BHP as substrates. Peroxidase activities were determined by recording NADPH oxidation at 340 nm. A control reaction in the absence of MstX was included. d–f The FOX assay of the MstX/MSH/Mtr pathway with H2O2, CHP, and t-BHP as substrates. The consumption of H2O2, CHP, and t-BHP by the FOX assay in function of time was shown. g–i The Michaelis–Menten plots of MstX activity versus H2O2, CHP, and t-BHP were performed with the reaction mixtures described in “Methods”. The data were presented as means of the values obtained from three independent assays. Kinetic parameters were calculated by non-linear regression using the program GraphPad Prism 5