Table 2 Primers for gene knockout used in this study
From: High ectoine production by an engineered Halomonas hydrothermalis Y2 in a reduced salinity medium
Name | Sequence |
|---|---|
ectD-F1 | CGCGGATCCATGACAGTCGGCAATACACCTCTC |
ectD-R1 | AAGGCAGGGCACCCGCTCGCGGTAAGCAT |
ectD-F2 | TTACCGCGAGCGGGTGCCCTGCCTTGGTGAAA |
ectD-R2 | CCCAAGCTTTTAAACGCCCGGCTGCCAAG |
doeA-F1 | CGCGGATCCGTGGCCCAGTGGCGCGTTTCA |
doeA-R1 | TTGAACACCCCGCCGGTGTGCCAGCCAC |
doeA-F2 | TGGCACACCGGCGGGGTGTTCAAGCGCTA |
doeA-R2 | CCCAAGCTTCTACTTCACGAACAGCTG |