Fig. 5

Predicted and experimental fluxes of key metabolites in the wild-type strain (SJ) and the adhE mutant. The wild-type strain was grown on glucose (a) and glucose and glycerol (b), and the adhE mutant was also grown on glucose (c) and glucose and glycerol (d). Bar plots show the average measured rates from 3 replicates (light orange), predicted rates from model constrained with average experimental uptake rates of the WT grown on glucose, or case 1 (dark green), and predicted rates from model constrained with average experimental rates from the strain and condition under study, or case 2 (light green). Metabolic maps show predicted flux distributions for case 1. All units for uptake- and secretion rates are in mmol gDW⁻¹ h⁻¹ and for growth rates in h⁻¹. Metabolic maps correspond to Fig. 1. Amino acids (a.a.) in case 1 refer to Arg, Ser, Asn, Asp and Glu