Fig. 4

Building the design of P. putida KT2440 capable of growth under anoxic conditions and testing under oxic conditions. a Schematic overview of constructs for P. putida KT2440: 2213F− (empty plasmid control), pS2213 ackA, pS2213 pyrK-pyrD B, pS2213 nrdD-nrdG and pS2213 ackA-(pyrK-pyrD B)-(nrdD-nrdG). The symbols in front of the strain names function as the legend for the graphs. b Aerobic growth analysis of all strains. c Aerobic fluorescence assay of all strains at excitation of 450 and emission of 495. d Fluorescence relative to cell density. Measurements for b, c and d were taken every 30 min for 64 h at \(30^\circ \text {C}\). Cultures were shaken continuously