Repression of mitochondrial metabolism for cytosolic pyruvate-derived chemical production in Saccharomyces cerevisiae

Table 1 Yeast strains and plasmids used in this study

Strain name	Description	Source or references
BY4742	MATα, his3Δ1, leu2Δ0, lys2Δ0, ura3Δ0	Thermo Scientific
mpc1Δ	BY4742 mpc1Δ::kanMX4	Thermo Scientific
atg32Δ	BY4742 atg32Δ:: kanMX4	Thermo Scientific
SCM001	BY4742/pGK426	This study
SCM053	mpc1Δ/pGK426	This study
SCM003	atg32Δ/pGK426	This study
SCM043	BY4742/pATP426-als^Lp_Op-aldc^Ll_Op-BDH1	This study
SCM051	mpc1Δ/pATP426-als^Lp_Op-aldc^Ll_Op-BDH1	This study
SCM042	atg32Δ/pATP426-als^Lp_Op-aldc^Ll_Op-BDH1	This study

Plasmid	Description	Source or reference
pGK426	Yeast expression vector containing PGK1 promoter, 2μ origin, URA3 marker, no expression (control plasmid)	Ishii et al. [33]
pATP426	Yeast three gene expression vector containing ADH1, TDH3, and PGK1 promoters, 2µ origin, URA3 marker, no expression.	Ishii et al. [34]
pATP426-als^Lp_Op-aldc^Ll_Op-BDH1	pATP426, co-expression of codon-optimized L. plantarum ALS, L. lactis ALDC and S. cerevisiae BDH1 genes controlled by ADH1, TDH3, and PGK1 promoters	Ishii et al. [6]

ISSN: 1475-2859