Skip to main content

Advertisement

Table 2 Strains and plasmids used in this study

From: Exploring novel herbicidin analogues by transcriptional regulator overexpression and MS/MS molecular networking

Strains/plasmids Relevant characteristics References
Strains
 Streptomyces mobaraensis US-43 Wild-type strain (herbicidin F-producing strain) Our laboratory
 US-43/pL-hcdR1 S. mobaraensis US-43 with the expression vector pL-hcdR1, Amr This work
 US-43/pL-hcdR2 S. mobaraensis US-43 with the expression vector pL-hcdR2, Amr This work
 US-43/pL-hcdR3 S. mobaraensis US-43 with the expression vector pL-hcdR3, Amr This work
 Escherichia coli
  DH5α General cloning host [34]
  ET12567/pUZ8002 Donor strain for intergeneric conjugation between E. coli and Streptomyces, Cmr, Kmr [35]
Plasmids
 pSET152 Streptomyces integrative vector, Amr [20]
 pL646 pSET152 derivative containing the constitutive promoter ermEp*, Amr [21]
 pL-hcdR1 pL646 derivative plasmid containing 684 bp complete coding region of hcdR1, Amr This work
 pL-hcdR2 pL646 derivative plasmid containing 2793 bp complete coding region of hcdR2, Amr This work
 pL-hcdR3 pL646 derivative plasmid containing 786 bp complete coding region of hcdR3, Amr This work
 pET16b-HcdR2 pET16b derivative plasmid containing 2793 bp complete coding region of hcdR2, Ampr This work
  1. Amr, apramycin resistance; Kmr, kanamycin resistance; Cmr, chloramphenicol resistance; Ampr, ampicillin resistence