Fig. 5

Pathway expression plasmid for GABA production was introduced into ST1, ST2, and ST8 (STG1, STG2, STG8, respectively). The strains were cultivated in flasks containing 2X M9 medium. a The growth profile, b acetate production, and c GABA production of STG1, STG2, and STG8 were monitored. Data of STG1, STG2, and STG8 are represented by black, red and blue lines, respectively. d The specific yield of GABA was calculated in the early stationary phase of cultivation. The samples of STG1, STG2, and STG8 were taken at 12 h, 24 h, and 24 h, respectively to calculate specific yield of GABA. All data are averaged through three independent experiments