Fig. 4

EGFP expression plasmid was introduced into ST1, ST2, and ST8 (STE1, STE2, STE8, respectively). The strains were cultivated in flasks containing 2X M9 medium. a The growth profile and (b) acetate production of STE1, STE2, and STE8 are presented. c The fluorescence intensity of strains monitored by microplate reader (excitation: 479 nm, emission: 520 nm). Data of STE1, STE2, and STE8 are represented by black, red, and blue lines, respectively. d The specific yield of EGFP was measured in the early stationary phase of cultivation. The samples of STE1, STE2, and STE8 were taken at 12 h, 24 h, and 24 h, respectively to calculate specific yield of EGFP. All data are averaged through three independent experiments