Optimization of Saccharomyces cerevisiae α-galactosidase production and application in the degradation of raffinose family oligosaccharides

Table 2 Summary of the purification of ScAGal

Purification step		U^a	mg	U/mg	PF^b	Yield
Purification step		U^a	mg	U/mg	PF^b	(U, %)	(mg, %)
Extracellular medium	1000 mL	3530	280	12.61	1.0	100	–
Concentrated medium	100 mL	2598	160	16.23	1.3	74	–
Affinity chromatography	10 mL	454	3.5	129.60	10.3	13	100
Dialysis + concentration	1 mL	238	3.2	74.55	–	7	91
Lyophilisation^c	169 mg	207	2.8	73.85	–	6	80

^aExtracellular α-galactosidase activity was determined using PNPG as substrate (40 °C, pH 4). ^bPurification factor. ^cmg dry weight

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