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Table 2 Summary of the purification of ScAGal

From: Optimization of Saccharomyces cerevisiae α-galactosidase production and application in the degradation of raffinose family oligosaccharides

Purification step   Ua mg U/mg PFb Yield
(U, %) (mg, %)
Extracellular medium 1000 mL 3530 280 12.61 1.0 100
Concentrated medium 100 mL 2598 160 16.23 1.3 74
Affinity chromatography 10 mL 454 3.5 129.60 10.3 13 100
Dialysis + concentration 1 mL 238 3.2 74.55 7 91
Lyophilisationc 169 mg 207 2.8 73.85 6 80
  1. aExtracellular α-galactosidase activity was determined using PNPG as substrate (40 °C, pH 4). bPurification factor. cmg dry weight