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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Optimization of Saccharomyces cerevisiae α-galactosidase production and application in the degradation of raffinose family oligosaccharides

Fig. 6

HPLC analysis of Gal1Man3 (a), Gal3.4Man5 (b) and locust bean gum (c) hydrolysed by ScAGal. Identification of the products obtained after digestion with β-mannosidase at 0 and 24 h of reaction (1 and 2), and after the action of ScAGal (0.3 U/ml in McIlvaine buffer, pH 4) for 4 and 24 h of the samples previously treated with β-mannosidase (3 and 4). G1M3, Gal1Man3; G1M2, Gal1Man2; GM, Gal1Man1; G3.4M5, Gal3.4Man5; G3.4M4, Gal3.4Man4; M, Mannose; X, Mannose and Galactose; mV, millivolts; Rt, retention time

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