Fig. 6

HPLC analysis of Gal¹Man₃ (a), Gal^3.4Man₅ (b) and locust bean gum (c) hydrolysed by ScAGal. Identification of the products obtained after digestion with β-mannosidase at 0 and 24 h of reaction (1 and 2), and after the action of ScAGal (0.3 U/ml in McIlvaine buffer, pH 4) for 4 and 24 h of the samples previously treated with β-mannosidase (3 and 4). G¹M₃, Gal¹Man₃; G¹M₂, Gal¹Man₂; GM, Gal¹Man₁; G^3.4M₅, Gal^3.4Man₅; G^3.4M₄, Gal^3.4Man₄; M, Mannose; X, Mannose and Galactose; mV, millivolts; Rt, retention time