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Table 2 Emulsifying activity (EI48) toward various hydrophobic substrates with AXE at different concentrations

From: Designer bioemulsifiers based on combinations of different polysaccharides with the novel emulsifying esterase AXE from Bacillus subtilis CICC 20034

Oil/hydrophobic AXE concentration (µg/ml)
500 200 100 50
n-Hexane 61.90 ± 0.56 48.10 ± 3.02 28.57 ± 2.39 14.29 ± 2.56
n-Hexadecane 9.52 ± 1.28 4.77 ± 0.78 4.77 ± 0.56 0
n-Octane 61.90 ± 0.92 52.38 ± 0.56 23.81 ± 2.37 19.05 ± 1.47
Benzene 66.67 ± 1.21 66.67 ± 0.77 66.13 ± 1.66 42.33 ± 1.84
Xylene 66.67 ± 0.81 66.67 ± 1.21 54.76 ± 1.66 28.57 ± 0.91
Kerosene 47.62 ± 1.84 47.62 ± 1.59 27.62 ± 1.07 23.81 ± 1.33
Diesel 66.67 ± 0.93 61.90 ± 1.77 23.81 ± 2.82 0
Soybean oil 23.81 ± 1.73 23.81 ± 2.54 14.29 ± 1.85 0
Lubricating oil 19.05 ± 2.98 4.76 ± 0.77 4.76 ± 1.98 4.76 ± 1.33
Olive oil 31.90 ± 1.72 27.12 ± 2.04 27.67 ± 1.49 22.83 ± 2.26
  1. The reaction was performed in identical cylindrical glass vials with a reaction volume of 2 ml purified AXE solution in 50 mM Tris–HCl buffer, pH 7.4, and 2 ml oil or hydrophobic substrates after vortexing. All samples were stored for 2 days before observation
  2. The results represent the mean ± SD over at least three replicates
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