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Table 2 Emulsifying activity (EI48) toward various hydrophobic substrates with AXE at different concentrations

From: Designer bioemulsifiers based on combinations of different polysaccharides with the novel emulsifying esterase AXE from Bacillus subtilis CICC 20034

Oil/hydrophobic

AXE concentration (µg/ml)

500

200

100

50

n-Hexane

61.90 ± 0.56

48.10 ± 3.02

28.57 ± 2.39

14.29 ± 2.56

n-Hexadecane

9.52 ± 1.28

4.77 ± 0.78

4.77 ± 0.56

0

n-Octane

61.90 ± 0.92

52.38 ± 0.56

23.81 ± 2.37

19.05 ± 1.47

Benzene

66.67 ± 1.21

66.67 ± 0.77

66.13 ± 1.66

42.33 ± 1.84

Xylene

66.67 ± 0.81

66.67 ± 1.21

54.76 ± 1.66

28.57 ± 0.91

Kerosene

47.62 ± 1.84

47.62 ± 1.59

27.62 ± 1.07

23.81 ± 1.33

Diesel

66.67 ± 0.93

61.90 ± 1.77

23.81 ± 2.82

0

Soybean oil

23.81 ± 1.73

23.81 ± 2.54

14.29 ± 1.85

0

Lubricating oil

19.05 ± 2.98

4.76 ± 0.77

4.76 ± 1.98

4.76 ± 1.33

Olive oil

31.90 ± 1.72

27.12 ± 2.04

27.67 ± 1.49

22.83 ± 2.26

  1. The reaction was performed in identical cylindrical glass vials with a reaction volume of 2 ml purified AXE solution in 50 mM Tris–HCl buffer, pH 7.4, and 2 ml oil or hydrophobic substrates after vortexing. All samples were stored for 2 days before observation
  2. The results represent the mean ± SD over at least three replicates
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Microbial Cell Factories

ISSN: 1475-2859

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