Fig. 5
From: Using a marine microalga as a chassis for polyethylene terephthalate (PET) degradation
UHPLC analysis of the medium fraction of shredded PET incubated with PETase-FLAG producing clone 2. The experiment was performed in 50 ml f/2-medium containing AP_SP-PETaseR280A-FLAG expressing clone 2 (AP2) and approximately 5 g of shredded PET. At T0 the cultures were adjusted to an OD600 of 0.4 and expression of the recombinant protein (AP_SP-PETaseR280A-FLAG) was induced with nitrate. Samples of 1 ml were taken at individual time points (T, 1 day = 24 h) and fresh nitrate was supplemented to the cultures at T3, T6 and T10. The standards used for UHPLC analyses are shown on the lower right. Note that the concentrations of standard compounds are not equal. BHET bis(2-hydroxyethyl) terephthalic acid, MHET mono(2-hydroxyethyl) terephthalic acid, TPA terephthalic acid, WT wild type, AP2 AP_SP-PETaseR280A-GFP clone 2