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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Using a marine microalga as a chassis for polyethylene terephthalate (PET) degradation

Fig. 3

Scanning electron microscopic analysis of PET bottle film degradation by PETase-FLAG secreted from P. tricornutum. As depicted in the upper left part, untreated PET and PET incubated with wild type cells on an f/2 agar plate overflowed with 2 ml f/2 liquid medium showed, besides a usually smooth surface, occasional stress marks. In contrast, PET incubated for 5 weeks with cells expressing AP_SP-PETaseR280A-FLAG (clone 1), was lanced by holes, dents, furrows and cavities when inspected via SEM (see also Additional file 1: Figure S5). In a specific area of the PET disk a structure (imprint) similar to the form of a P. tricornutum cell (fusiform morphotype) from which several holes and furrows originated was detected (see also Additional file 1: Figure S6). AP alkaline phosphatase, SP signal peptide, WT wild type, AP_1 AP_SP-PETaseR280A-GFP clone 1

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