Table 1 List of Z. mobilis strains used in this study
From: Establishment and application of a CRISPR–Cas12a assisted genome-editing system in Zymomonas mobilis
Strains | Description | Note |
|---|---|---|
Z. mobilis ZM4 | Z. mobilis subsp. mobilis ZM4 (wild-type strain) | ATCC31821 |
ZM-Cas12a | The Cas12a expressing cassette with spectinomycin resistance integrated into ZMO0038 locus | This study |
ZM-Δ0028 | ZM-Cas12a with deletion of ZMO0028 | This study |
ZM-ΔHfq | ZM-Cas12a with deletion of ZMO0347 | This study |
ZM-mCherry | ZMO0028 replaced by the reporter gene mCherry | This study |
ZM-LdhBc | ZMO0028 replaced by gene LdhBc | This study |
ZM-Cas12a-ΔpZM33 | ZM-Cas12a with native plasmid pZM33 cured | This study |
ZM-Cas12a-ΔpZM36 | ZM-Cas12a with native plasmid pZM36 cured | This study |
ZM-Cas12a-ΔpZM39 | ZM-Cas12a with native plasmid pZM39 cured | This study |