Fig. 2
From: Establishment and application of a CRISPR–Cas12a assisted genome-editing system in Zymomonas mobilis
Native plasmid curing in Z. mobilis by CRISPR–Cas12a system and the impact of plasmid curing on cellular growth. The native plasmid curing was identified by colony PCR. Primers specific for native plasmids of pZM33 (P33-Check-F/R, 1192 bp), pZM36 (P36-Check-F/R, 896 bp) and pZM39 (P39-Check-F/R, 1121 bp) were used to verify the existence of each plasmid, respectively (a). The growth curve of Z. mobilis and plasmid-deleted mutants using Bioscreen C (b)