Fig. 1
From: Establishment and application of a CRISPR–Cas12a assisted genome-editing system in Zymomonas mobilis
Scheme and genome editing using the heterologous CRISPR–Cas12a system. Scheme of genome editing with Cas12a using a single plasmid approach. Tetracycline-inducible Cas12a is directed to specific DNA targets by constitutively expressed sgRNAs. Cas12a was stably integrated into the ZMO0038 locus, and the assistant plasmid constitutively express sgRNAs (a). Colony forming unit (c.f.u) of Z. mobilis ZM4 and the Cas12a expressing strain with or without the expression of crRNA targets (b). Genome editing mediated by Cas12a with different versions of artificial CRISPR arrays. Array 1 crRNAs are in a double direct repeat form (19-nt DR with 23-nt guide); Array 2 crRNAs are in a single direct repeat form (35-nt DR with 19-nt guide); and Array 3 crRNAs are in their mature form (19-nt DR with 23-nt guide) (c). c.f.u of Z. mobilis expressing the nuclease with different versions of artificial CRISPR arrays (d). Values are the means of experiments with three or more technical replicates; error bars are standard deviations