Efficient expression vectors and host strain for the production of recombinant proteins by Yarrowia lipolytica in process conditions

Table 3 Dynamics of CalB production for DASGIP bioreactor cultures of strains RIY368 (mono-copy) and RIY406 (multi-copy)

Parameters	Strains
Parameters	RIY368	RIY406
Specific cell growth rate (h⁻¹)	0.29 ± 0.00	0.27 ± 0.01
Specific glycerol uptake rate (g gDCW⁻¹ h⁻¹)	0.37 ± 0.06	0.44 ± 0.00
Maximum lipase activity (U mL⁻¹)	28,024 ± 743	45,125 ± 2144
Lipase volumetric production rate (U mL⁻¹ h⁻¹)	1357 ± 34	2179 ± 104

Cells were grown for 48 h at 28 °C in YNBG₂E medium, in DASGIP bioreactors. Displayed means and standard deviations are the result of duplicate experiments

ISSN: 1475-2859