Biosynthesis of rhamnolipid by a Marinobacter species expands the paradigm of biosurfactant synthesis to a new genus of the marine microflora

Table 1 Phylogenetic identification and biosurfactant phenotypic screening results for each bacterial strain

Strain	Origin	BLASTn identification (against NCBI database)	GeneBank accession number	Sequence similarity (%)	Phenotypic screening
Strain	Origin	BLASTn identification (against NCBI database)	GeneBank accession number	Sequence similarity (%)	ST (mN m⁻¹)^a	EI_{24 h} (%)^b
MCTG106	Coastal surface water, Oregon, Washington State, USA	Marinobacter sp. NP1383C-30R	MK894600	100	54.63 ± 3.5	40 ± 1.5
MCTG4b	Laboratory culture of Thalassiosira weissflogii strain CCMP 1052 isolated from Oslo Fjord, Norway	Marinobacter sp. Set72	MK894835	99	38.5 ± 0.6	42 ± 2.0
MCTG167	Phytoplankton net tow, Oban, UK	Marinobacter sp. T23	MK894854	100	61.55 ± 0.1	N/A
MCTG161(2c3)	Phytoplankton net tow, Oban, UK	Marinobacter adhaerens HP15	MK894872	99	60.0 ± 0.5	45 ± 2.0
MCTG107b	Coastal surface water, Oregon, Washington State, USA	Marinobacter sp. R-28768	MK578516	100	31.0 ± 0.5	40 ± 1.8

Bacteria were identified by 16S rDNA gene sequencing. Surface tension values (mN m⁻¹) and EI₂₄ (%) were obtained from cell-free supernatant samples of cultures incubated for 96 h
^aST and ^bEI 24 h of sterile ZM/1 medium was 58 mNm⁻¹ and 0%, respectively

ISSN: 1475-2859