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Table 1 Bacterial strains and plasmids used in this study

From: Mevalonate production from ethanol by direct conversion through acetyl-CoA using recombinant Pseudomonas putida, a novel biocatalyst for terpenoid production

Strain, plasmid Genotype or properties Source
Strains
 P. putida KT2440 Wild-Type ATCC
 E. coli DH10B F-endA1recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ-T1R RBC
 ELPP000 P. putida KT2440 harboring pSGP10, pAWP89-0 This study
 ELPP010 P. putida KT2440 harboring pSGP11, pAWP89-0 This study
 ELPP0dT0 P. putida KT2440 harboring pSGP1dT, pAWP89-0 This study
 ELPP1dT0 P. putida KT2440 ΔendA ΔendX harboring pSGP1dT, pAWP89-0 This study
 ELPP110 P. putida KT2440 ΔendA ΔendX harboring pSGP11, pAWP89-0 This study
 ELPP111 P. putida KT2440 ΔendA ΔendX harboring pSGP11, pAWP89-1 This study
 ELPP211 P. putida KT2440 ΔendA ΔendX ΔqedH-I ΔqedH-II harboring pSGP11, pAWP89-1 This study
 ELPP212 P. putida KT2440 ΔendA ΔendX ΔqedH-I ΔqedH-II harboring pSGP11, pAWP89-2 This study
 ELPP213 P. putida KT2440 ΔendA ΔendX ΔqedH-I ΔqedH-II harboring pSGP11, pAWP89-3 This study
 ELPP221 P. putida KT2440 ΔendA ΔendX ΔqedH-I ΔqedH-II harboring pSGP12, pAWP89-1 This study
 ELPP311 P. putida KT2440 ΔendA ΔendX ΔqedH-I ΔqedH-II ΔphaG harboring pSGP11, pAWP89-1 This study
Plasmids
 pUCP19 E. coli–Pseudomonas Shuttle Expression Vector, Plac, AmpR ATCC
 pUC19 E. coli Expression Vector, lacI, Plac, AmpR Takara
 pTrc99A E. coli Expression Vector, lacI, Ptrc, AmpR [24]
 pCM184 Allelic Exchange Vector, AmpR, TetR, loxP-KanR-loxP [25]
 pAWP89 Broad Host Range Expression Vector, Ptac-dTomato, KanR [26]
 pK19mobsacB Allelic Exchange Vector, sacB, KanR [27]
 pK19mobsacB (::lacI) pK19mobsacB-lacI (derived from pTrc99A) This Study
 pSGP10 E. coli-Pseudomonas Shuttle Expression Vector (pUCP19 derived), lacI + Ptrc derived from pTrc99A, TetR derived from pCM184 This Study
 pSGP1dT pSGP10-dTomato (derived from pAWP89) This Study
 pSGP11 pSGP10-mvaE_opti-mvaS_opti-atoB_opti This Study
 pSGP12 pSGP10-mvaE_opti-mvaS_opti-atoB_opti-nphT7_opti This Study
 pAWP89-0 Broad Host Range Expression Vector (pAWP89 derived), Plac drived from pUC19, MCS from MEVa Vector This Study
 pAWP89-1 pAWP89-0-acs_opti This Study
 pAWP89-2 pAWP89-0-eutE_opti This Study
 pAWP89-3 pAWP89-0-acs_opti-eutE_opti This Study
  1. aDedicated by professor SEON-WON Kim in Gyeongsang National University