Table 1 Bacterial strains and plasmids used in this study

Strains

 P. putida KT2440

Wild-Type

ATCC

 E. coli DH10B

F-endA1recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ-T1R

RBC

 ELPP000

P. putida KT2440 harboring pSGP10, pAWP89-0

This study

 ELPP010

P. putida KT2440 harboring pSGP11, pAWP89-0

This study

 ELPP0dT0

P. putida KT2440 harboring pSGP1dT, pAWP89-0

This study

 ELPP1dT0

P. putida KT2440 ΔendA ΔendX harboring pSGP1dT, pAWP89-0

This study

 ELPP110

P. putida KT2440 ΔendA ΔendX harboring pSGP11, pAWP89-0

This study

 ELPP111

P. putida KT2440 ΔendA ΔendX harboring pSGP11, pAWP89-1

This study

 ELPP211

P. putida KT2440 ΔendA ΔendX ΔqedH-I ΔqedH-II harboring pSGP11, pAWP89-1

This study

 ELPP212

P. putida KT2440 ΔendA ΔendX ΔqedH-I ΔqedH-II harboring pSGP11, pAWP89-2

This study

 ELPP213

P. putida KT2440 ΔendA ΔendX ΔqedH-I ΔqedH-II harboring pSGP11, pAWP89-3

This study

 ELPP221

P. putida KT2440 ΔendA ΔendX ΔqedH-I ΔqedH-II harboring pSGP12, pAWP89-1

This study

 ELPP311

P. putida KT2440 ΔendA ΔendX ΔqedH-I ΔqedH-II ΔphaG harboring pSGP11, pAWP89-1

This study

Plasmids

 pUCP19

E. coli–Pseudomonas Shuttle Expression Vector, P_lac, Amp^R

ATCC

 pUC19

E. coli Expression Vector, lacI, P_lac, Amp^R

Takara

 pTrc99A

E. coli Expression Vector, lacI, P_trc, Amp^R

[24]

 pCM184

Allelic Exchange Vector, Amp^R, Tet^R, loxP-Kan^R-loxP

[25]

 pAWP89

Broad Host Range Expression Vector, P_tac-dTomato, Kan^R

[26]

 pK19mobsacB

Allelic Exchange Vector, sacB, Kan^R

[27]

 pK19mobsacB (::lacI)

pK19mobsacB-lacI (derived from pTrc99A)

This Study

 pSGP10

E. coli-Pseudomonas Shuttle Expression Vector (pUCP19 derived), lacI + P_trc derived from pTrc99A, Tet^R derived from pCM184

This Study

 pSGP1dT

pSGP10-dTomato (derived from pAWP89)

This Study

 pSGP11

pSGP10-mvaE_opti-mvaS_opti-atoB_opti

This Study

 pSGP12

pSGP10-mvaE_opti-mvaS_opti-atoB_opti-nphT7_opti

This Study

 pAWP89-0

Broad Host Range Expression Vector (pAWP89 derived), P_lac drived from pUC19, MCS from MEV^a Vector

This Study

 pAWP89-1

pAWP89-0-acs_opti

This Study

 pAWP89-2

pAWP89-0-eutE_opti

This Study

 pAWP89-3

pAWP89-0-acs_opti-eutE_opti

This Study