Cooperation of hydrolysis modes among xylanases reveals the mechanism of hemicellulose hydrolysis by Penicillium chrysogenum P33

Table 3 Reducing sugars (mg/mL) released by individual and combined of the three wild-type xylanases against delignified corn stover

Enzymes	6 h	12 h	24 h	48 h
Xyl1	0.007 ± 0.001^a	0.007 ± 0.000^A	0.002 ± 0.000^α	0.014 ± 0.001ⁱ
Xyl2	0.028 ± 0.009^ab	0.049 ± 0.019^AB	0.036 ± 0.018^αβ	0.033 ± 0.013ⁱ
Xyl3	0.041 ± 0.001^ab	0.138 ± 0.011^CD	0.212 ± 0.020^γ	0.345 ± 0.016ⁱⁱⁱ
Xyl1 + Xyl2	0.055 ± 0.025^b	0.085 ± 0.037^BC	0.063 ± 0.023^β	0.082 ± 0.025ⁱⁱ
Xyl1 + Xyl3	0.057 ± 0.008^b	0.153 ± 0.004^D	0.256 ± 0.019^γ	0.369 ± 0.004ⁱⁱⁱ
Xyl2 + Xyl3	0.327 ± 0.024^c	0.442 ± 0.034^E	0.541 ± 0.020^δ	0.652 ± 0.033^iv
Xyl1 + Xyl2 + Xyl3	0.345 ± 0.012^c	0.495 ± 0.015^E	0.588 ± 0.025^δ	0.705 ± 0.006^v

The experiments were performed in triplicate, and the data are presented as the means ± standard deviations
Statistical significance among the data in the same column is indicated by different letters as assessed by ANOVA (Duncan test, p < 0.05)

ISSN: 1475-2859