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Table 1 The specific activities and kinetic parameters of the purified recombinant xylanases

From: Cooperation of hydrolysis modes among xylanases reveals the mechanism of hemicellulose hydrolysis by Penicillium chrysogenum P33

Enzymes

Specific activity (U/mg)

Vmax (μmol/min/mg)

Km (mg/mL)

kcat (s−1)

kcat/Km (mL/mg/s)

Xyl1

151.5 ± 4.7

327.2 ± 13.29

9.6 ± 1.2

180.2 ± 7.3

18.7 ± 0.8

Xyl2

2149.8 ± 43.1

2779 ± 101.5

3.8 ± 0.5

990.7 ± 36.2

260.5 ± 9.6

Xyl3

290.6 ± 7.9

392.4 ± 51.08

4.8 ± 1.4

262.1 ± 34.1

54.7 ± 7.1

  1. Reactions were performed at each corresponding optimal conditions
  2. The experiments were performed in duplicate, and the data are presented as the means ± standard deviations
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