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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Cooperation of hydrolysis modes among xylanases reveals the mechanism of hemicellulose hydrolysis by Penicillium chrysogenum P33

Fig. 6

Hydrolysis of beechwood xylan by the recombinant xylanases. a Individual and combined enzymatic hydrolysis of beechwood xylan. M, Size markers for xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4) and xylopentaose (X5); 1, control no digested samples; 2, Xyl1; 3, Xyl2; 4, Xyl3; 5, Xyl1 + Xyl2; 6, Xyl1 + Xyl3; 7, Xyl2 + Xyl3; 8, and Xyl1 + Xyl2 + Xyl3; b Sequential hydrolysis of beechwood xylan. M, Size markers for X1, X2, X3, X4 and X5; 1, control no digested samples; 2, 6 and 10, hydrolysis with Xyl1, Xyl2 and Xyl3 alone for 2 h, respectively; 3, 4 and 5, hydrolysis with Xyl1 for 2 h following supplementation with Xyl1, Xyl2 and Xyl3 for another 4 h, respectively; 7, 8 and 9, hydrolysis with Xyl2 for 2 h following supplementation with Xyl2, Xyl1 and Xyl3 for another 4 h, respectively; 11, 12 and 13, hydrolysis with Xyl3 for 2 h following supplementation with Xyl3, Xyl1 and Xyl2 for another 4 h, respectively

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