Table 2 Strains used in this study
From: Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory
Strains | Description | Parental strain |
|---|---|---|
W303-1a | MATa; leu2-3,112; trp1-1; can1-100; ura3-1; ade2-1; his3-11,15 | – |
LCB01 | δ::Ppgk1-tHMG1-Tpgk1-PTDH3-IDI1-TADH1 | W303-1a |
LCB02 | δ::Ppgk1-tHMG1-Tpgk1-PTDH3-IDI1-TADH1; Pxp320-tVvTS | LCB01 |
LCB03 | δ::Ppgk1-tHMG1-Tpgk1-PTDH3-IDI1-TADH1; erg20::Ppgk1-ERG20(F96W-N127W); Pxp320-tVvTS | LCB02 |
LCB04 | δ::Ppgk1-tHMG1-Tpgk1-PTDH3-IDI1-TADH1; erg20::Ppgk1-ERG20(F96W-N127W-K197G); Pxp320-tVvTS | LCB02 |
LCB05 | δ::Ppgk1-tHMG1-Tpgk1-PTDH3-IDI1-TADH1; erg20::Ppgk1-ERG20(F96W-N127W); Pmet3-erg9; Pxp320-tVvTS | LCB03 |
LCB06 | δ::Ppgk1-tHMG1-Tpgk1-PTDH3-IDI1-TADH1; erg20::Ppgk1-ERG20(F96W-N127W); Pmet3-erg9; LPP1:: loxp; DPP1::loxp; Pxp320-tVvTS | LCB05 |
LCB07 | δ::Ppgk1-tHMG1-Tpgk1-PTDH3-IDI1-TADH1; erg20::Ppgk1-ERG20(F96W-N127W); rDNA::Ppgk1-ERG20(F96W-N127W)-(GS)5-tVvTS-TADH1; Pxp320-tVvTS | LCB03 |
LCB08 | δ::Ppgk1-tHMG1-Tpgk1-PTDH3-IDI1-TADH1; erg20::Ppgk1-ERG20(F96W-N127W); rDNA::Ppgk1-ERG20(F96W-N127W)-(GS)5-tVvTS-TADH1; HO::PTDH3-ERG9-TADH1;Pxp320-tVvTS | LCB07 |