Fig. 7
From: Improvement of Fab expression by screening combinatorial synonymous signal sequence libraries
Correlation between positional Gibbs free energy of mRNA secondary structure and Fab expression in the light chain region of the N-0 library. Black line indicates the p-value at given position and dashed black line indicates the level below which the correlation is significant. Grey line indicates the Spearman rank correlation coefficient value at given position. The grey arrow denotes the start and the translational direction of the light chain PelB. Spearman rank correlation coefficients (r), p-values and sampling sizes (n) of each correlation analysis at each position are shown in numerical form below the graph